| distance | reference number | name | latitude | longitude |
| 45.0 | 1028 | Fort Myers, Caloosahatchee River, Florida | 26.6467 | -81.8717 |
| 45.0 | 1029 | Fort Myers, Florida | 26.6467 | -81.8717 |
| 47.1 | 603 | Coconut Point, Estero Bay, Florida | 26.4 | -81.8333 |
| 47.7 | 935 | Estero Bay, Estero River, Florida | 26.43 | -81.8567 |
| 49.1 | 602 | Cocohatchee River, U.S. 41 bridge, Florida | 26.2817 | -81.8017 |
| 49.4 | 1647 | Little Hickory Island, Estero Bay, Florida | 26.35 | -81.85 |
| 49.5 | 429 | Cape Coral Bridge, Florida | 26.5667 | -81.9333 |
| 49.7 | 3340 | Wiggins Pass, Cocohatchee River, Florida | 26.29 | -81.8183 |
| 49.9 | 465 | Carlos Point, Estero Bay, Florida | 26.4 | -81.8833 |
| 52.1 | 1389 | Iona Shores, Caloosahatchee River, Florida | 26.5167 | -81.9667 |
| 52.4 | 1777 | Matanzas Pass (fixed bridge) Estero Island, Florida | 26.45 | -81.95 |
| 54.0 | 1943 | Naples, Naples Bay, north end, Florida | 26.1367 | -81.7883 |
| 54.1 | 2773 | Shell Point, Peace River, Florida | 26.9833 | -82 |
| 55.0 | 1942 | Naples, Gulf Of Mexico, Florida | 26.13 | -81.8067 |
| 55.6 | 2472 | Punta Rassa, San Carlos Bay, Florida | 26.4833 | -82.0167 |
| 55.6 | 946 | Everglades City, Barron River, Florida | 25.8583 | -81.3867 |
| 55.9 | 2466 | Pumpkin Bay, Florida | 25.9167 | -81.55 |
| 56.2 | 2333 | Point Ybel, San Carlos Bay Entrance, Florida | 26.45 | -82.0167 |
| 56.7 | 1784 | Matlacha Pass (bascule bridge), Florida | 26.6333 | -82.0667 |
| 57.2 | 2032 | North Fork, St. Lucie River, Florida | 27.2433 | -80.3133 |
| 57.3 | 2469 | Punta Gorda, Charlotte Harbor, Florida | 26.9333 | -82.0667 |
| 57.8 | 560 | Chokoloskee, Florida | 25.8133 | -81.3633 |
| 58.1 | 2863 | South Fork, St. Lucie River, Florida | 27.165 | -80.255 |
| 58.5 | 3135 | Tropical Homesites Landing, Pine Island, Florida | 26.55 | -82.0833 |
| 58.9 | 2974 | Stuart, St. Lucie River, Florida | 27.2 | -80.2583 |