| distance | reference number | name | latitude | longitude |
| 20.5 | 1698 | Lostmans River, Onion Key, Florida | 25.6167 | -81.1333 |
| 25.5 | 525 | Chatham River entrance, Florida | 25.6833 | -81.2833 |
| 26.9 | 1697 | Lostmans River entrance, Florida | 25.55 | -81.2167 |
| 28.0 | 560 | Chokoloskee, Florida | 25.8133 | -81.3633 |
| 29.2 | 946 | Everglades City, Barron River, Florida | 25.8583 | -81.3867 |
| 34.0 | 3333 | Whitewater Bay, Florida | 25.3167 | -81.0333 |
| 34.0 | 2754 | Shark River entrance, Florida | 25.35 | -81.1333 |
| 34.2 | 1379 | Indian Key, Florida | 25.8 | -81.4667 |
| 38.0 | 2593 | Round Key, Florida | 25.8333 | -81.5333 |
| 38.8 | 718 | Cutler, Biscayne Bay, Florida | 25.615 | -80.305 |
| 39.1 | 2466 | Pumpkin Bay, Florida | 25.9167 | -81.55 |
| 40.7 | 777 | Dinner Key Marina, Biscayne Bay, Florida | 25.7267 | -80.2367 |
| 42.9 | 3151 | Turkey Point, Biscayne Bay, Florida | 25.4367 | -80.3283 |
| 43.2 | 1821 | Miami, Marina, Biscayne Bay, Florida | 25.7783 | -80.185 |
| 43.5 | 996 | Flamingo, Florida Bay, Florida | 25.1417 | -80.9233 |
| 44.0 | 632 | Coon Key, Florida | 25.9 | -81.6333 |
| 44.2 | 262 | Biscayne Creek, ICWW, Florida | 25.88 | -80.1633 |
| 44.3 | 793 | Dodge Island, Fishermans Channel, Florida | 25.77 | -80.1683 |
| 44.4 | 2654 | San Marino Island, Florida | 25.7933 | -80.1633 |
| 45.0 | 1494 | Key Biscayne Yacht Club, Biscayne Bay, Florida | 25.6983 | -80.17 |
| 45.0 | 199 | Bear Cut, Virginia Key, Florida | 25.7317 | -80.1617 |
| 45.0 | 3230 | Virginia Key, Bear Cut, Biscayne Bay, Florida | 25.7317 | -80.1617 |
| 45.4 | 1376 | Indian Creek Golf Club, ICWW, Florida | 25.875 | -80.1433 |
| 46.3 | 464 | Card Sound, western side, Florida | 25.345 | -80.3317 |
| 46.3 | 452 | Cape Sable, East Cape, Florida | 25.1167 | -81.0833 |