| distance | reference number | name | latitude | longitude |
| 0.8 | 108 | Atlantic Beach, Florida | 30.335 | -81.395 |
| 2.6 | 2163 | Pablo Creek, ICWW bridge, St. Johns River, Florida | 30.3233 | -81.4383 |
| 3.0 | 1792 | Mayport Naval Station, Degausing Structure, St. Joh | 30.3967 | -81.395 |
| 3.3 | 1793 | Mayport Naval Station, Florida | 30.4 | -81.4133 |
| 3.3 | 3273 | Water Treatment Dock, Mayport Naval Station, St Joh | 30.4 | -81.4133 |
| 3.4 | 1791 | Mayport (ferry dock), Florida | 30.3933 | -81.4317 |
| 3.4 | 2162 | Pablo Creek entrance, St. Johns River, Florida | 30.3767 | -81.4483 |
| 3.5 | 149 | Bar Pilot Dock, St. Johns River, Florida | 30.3967 | -81.43 |
| 3.9 | 1405 | Jacksonville Beach, Florida | 30.2833 | -81.3867 |
| 5.0 | 1657 | Little Talbot Island, Florida | 30.43 | -81.405 |
| 5.3 | 2804 | Sisters Creek, St. Johns River, Florida | 30.4167 | -81.4533 |
| 5.9 | 2076 | Oak Landing, ICWW, Florida | 30.2533 | -81.43 |
| 6.0 | 1022 | Fort George Island, Fort George River, Florida | 30.44 | -81.4383 |
| 6.9 | 1063 | Fulton, St. Johns River, Florida | 30.39 | -81.5067 |
| 7.3 | 2802 | Simpson Creek, A1A highway bridge, Florida | 30.465 | -81.4317 |
| 7.5 | 577 | Clapboard Creek, Pelotes Island, St. Johns River, F | 30.4067 | -81.51 |
| 9.5 | 283 | Blount Island Bridge, St. Johns River, Florida | 30.4133 | -81.545 |
| 9.8 | 732 | Dame Point, Florida | 30.3867 | -81.5583 |
| 9.8 | 733 | Dame Point, St. Johns River, Florida | 30.3867 | -81.5583 |
| 10.3 | 2693 | Sawpit Creek, 1 mi. above entrance, Florida | 30.5033 | -81.4717 |
| 10.6 | 2692 | Sawpit Creek entrance, bridge, Florida | 30.5133 | -81.4567 |
| 10.8 | 2929 | St. Marys River (entrance), Florida | 30.5183 | -81.4533 |
| 10.9 | 3093 | Tiger Point, Pumpkin Hill Creek, St. Marys River, F | 30.5017 | -81.495 |
| 12.6 | 886 | Edwards Creek, 1 mi. above entrance, Florida | 30.5017 | -81.5417 |
| 12.7 | 1650 | Little Pottsburg Creek, St. Johns River, Florida | 30.31 | -81.61 |